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Publication details

Automatic Determination of the Size of Elliptical Nanoparticles from AFM Images

Journal Article

Sedlář Jiří, Zitová Barbara, Kopeček Jaromír, Flusser Jan, Todorcius T., Kratochvílová Irena

serial: Journal of Nanoparticle Research vol.15, 8 (2013), p. 1-10

project(s): GAP103/11/1552, GA ČR, GAP304/10/1951, GA ČR, TA01011165, GA TA ČR

keywords: Atomic force microscopy, Image moments, Pyrrole derivatives, Size determination, Watershed segmentation

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abstract (eng):

The objective of this work was to develop an accurate method for automatic determination of the size of elliptical nanoparticles from atomic force microscopy (AFM) images that would yield results consistent with results of manual measurements by experts. The proposed method was applied on phenylpyridyldiketopyrrolopyrrole (PPDP). A PPDP layer consists of similarly sized elliptical particles (c. 100 nm 9 50 nm) and its properties can be estimated from the average length and width of the particles. The developed method is based on segmentation of salient particles by the watershed transform and approximation of their shapes by ellipses computed by image moments; it estimates the lengths and widths of the particles by the major and minor axes, respectively, of the corresponding ellipses. Its results proved to be consistent with results of manual measurements by a trained expert. The comparison showed that the developed method could be used in practice for precise automatic measurement of PPDP particles in AFM images.


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Last modification: 21.12.2012
Institute of Information Theory and Automation